Affinity and beyond: The future of separation technologies
Protein A has been a mainstay of downstream processing for MAbs for more than two decades. Its use with affinity chromatography was particularly effective in capturing product from supernatants when protein titers were low. With improvements in expression systems producing dramatically higher titers, increasing pressure to reduce costs, and the advent of single-use technologies, new approaches to MAb separations are emerging. These slides were presented as part of Mr. Ransohoff’s introductory remarks for a panel which reviewed some of these methods and how they can compete with the legacy of Protein A and discussed how Protein A manufacturers can adapt to a more competitive environment for antibody separations technology.